Our research backs up this theory by demonstrating the difference in the levels of peripheral blood mononuclear cell (PBMC) subsets in canines’ PRF. Also, the microscopic appearance of the composition and distribution of PRF seems identical to what was detected in human research 1. The previous study in dogs and cats revealed that wound healing initiation components of platelet-rich fibrin (PRF), such as levels of different growth factors, are similar to humans 1. However, inflammatory and neoplastic processes do not affect the distribution of PBMC. Proportions of monocytes and macrophages were higher in PRF samples compared to the blood of healthy patients and periodontitis-affected patients. A higher proportion of PBMC cells related to wound healing (CD3+, CD3+ CD4+ CD8−, CD14+) were found in the PRF of control, periodontitis and neoplasia groups compared to the respective blood samples, which implies a positive outcome associated with clinical PRF usage in canine patients. In the present study, canine PRF cell composition was analyzed for the first-time using cell flow cytometry protocol. Individual clinical parameters of the patients and evaluation of the wound healing quality were included in the research.

The canine patient population was divided into three subgroups: animals with periodontitis only, animals with neoplasia and periodontitis, and healthy controls. The study aimed to supplement currently available data on PRF cell composition in canine patients by isolating peripheral blood mononuclear cells (PBMC), namely T cells, matured B cells, monocytes and macrophages, and adapting current protocols of cell flow cytometry for PRF analysis. However, data reporting about its application in companion animals is lacking. Platelet-rich fibrin (PRF) is a hot research topic because of its regenerative effect in humans.

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